Luria Bertani Medium 500 gram

L.B. MEDIUM (LURIA BERTANI)
Dehydrated medium for maintaining and cultivating of recombinant strains of Escherichia coli
TYPICAL FORMULA (g/L)
Tryptone............................................................................... 10.0
Yeast extract ……………..……………………………........... 5.0
Sodium chloride................................................................... 5.0
Final pH 7.0 ± 0.2
DESCRIPTION
L.B.MEDIUM (LURIA BERTANI) is a dehydrated medium used for maintaining and cultivating of recombinat strains of Escherichia coli in molecular biology procedures.These strains are generally derived from E.coli K12, which are deficient in B vitamin production. Thisstrain of E.coli has been further modification throught specific mutation to create an auxotrophic strain that is not capable of growth onnutrionally deficient media.
PRINCIPLE
L.B.MEDIUM (LURIA BERTANI) provides all the nutritional requirements of recombinat strains of Escherichia coli. Peptone providesnitrogen and carbon. Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Sodium ions for transportand osmotic balance are provided by sodium chloride.