Himedia Potato Dextrose Broth, Granulated GM403-500G Potato Dextrose Broth, granulated is recommended for the isolation and enumeration of yeasts and moulds . Composition** Ingredients Gms / Litre Potatoes, infusion from 200.000 Dextrose 20.000 Final pH ( at 25°C) 5.1±0.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 24.0 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.