Urea Broth Base (Diagnostic Stuarts Urea Broth Base) Himedia M111-500 Intended Use: Urea Broth is recommended for the identification of bacteria on the basis of urea utilization, specifically for the differentiation of Proteus species from Salmonella and Shigella species. Composition** Ingredients Gms / Litre Monopotassium phosphate 9.100 Dipotassium phosphate 9.500 Yeast extract 0.100 Phenol red 0.010 Final pH ( at 25°C) 6.8±0.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 18.71 grams in 950 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 55°C. Aseptically add 50 ml of sterile 40% Urea solution (FD048). Mix well and distribute in 10 ml amounts into sterile tubes.