Nutrient Broth Himedia M002 -500g Intended use Nutrient Broth is used for the general cultivation of less fastidious microorganisms , can be enriched with blood or other biological fluids. Composition** Ingredients Gms / Litre Peptone 5.000 Sodium chloride 5.000 HM peptone B# 1.500 Yeast extract 1.500 Final pH ( at 25°C) 7.4±0.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 13.0 grams in 1000 ml distilled water. Heat, if necessary, to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.