HiPurA DH5a Competent Cells 20app

Introduction: HiPurA Competent Cells are specially prepared chemically competent DH5α cells which makes cloning experiments easier. Competent cells are ready to use bacterial cells that contain an altered cell membrane through which a foreign DNA can pass through very easily. HiPurA Competent Cells are prepared by following a modified protocol of Hanahan (1983, J.Mol.Biol 166:557) and are recommended for regular subcloning of recombinant plasmids. DH5α is the most frequently used E.coli strain for routine cloning applications through blue/white screening of colonies on plates containing X-Gal and IPTG as the lacZ∆M15 marker provides α-complementation of the β-galactosidase gene. A stock pUC19 solution is provided as a control to determine the transformation efficiency. To obtain maximum transformation efficiency, the experimental DNA must be free of phenol, ethanol, protein and detergents. Genotype: F- φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk -, mk +) phoA supE44 thi-1 gyrA96 relA1 λ Applications: o Highest transformation efficiency o General cloning o Blue-white selection o Plasmid isolation