EC BROTH 500Gr | CM0853B OXOID

EC Broth 500Gr | Oxoid CM0853B Descriptions : A selective broth for the growth of coliforms and Escherichia coli from food and environmental samples. Directions Dissolve 37g in 1 litre of distilled water. Dispense into final containers and sterilize by autoclaving at 121C for 15 minutes. Description The original formulation described by Perry and Hajna1 with Tryptose as the nitrogen source was subsequently substituted for Tryptone . The medium has been shown to be a reliable medium producing dependable results in 24 to 48 hours2. EC Broth is a selective medium for the differentiation of faecal coliforms and the confirmatory test for Escherichia coli from food and environmental samples3,4. It is also suitable for the enumeration of presumptive E. coli in milk and milk products using the most probable number technique2. Escherichia coli is a common Gram-negative micro-organism which may be present in food and water samples. The presence of E. coli cells may be indicative of faecal contamination, although their ingestion does not necessarily have adverse effects on health. Nevertheless, certain strains are responsible for diarrhoeal disease and can also lead to more serious forms of illness. The presence of E. coli in water is also regarded as a specific indicator of faecal pollution, but lack of rapid identification methods means that the non-specific group - faecal coliforms - is also used as the indicator of such pollution. In this regard, it is important to differentiate between coliforms of faecal origin and those from other sources.