Violet Red Bile Glucose (VRBG) Agar (ISO) 500Gr | Oxoid CM1082B

Violet Red Bile Glucose (VRBG) Agar (ISO) 500Gr | Oxoid CM1082B Violet Red Bile Glucose Agar (VRBGA) (ISO) is for the detection and enumeration of Enterobacteriaceae in food, animal feed and environmental samples. This medium meets the formulation and performance criteria laid down in ISO 215281.2 and ISO 11133:20143. Directions Suspend 38.5g of VRBGA (ISO) dehydrated medium in 1 litre of distilled water. Bring to the boil while mixing. Continue to boil for up to 2 minutes or for the minimum time necessary to dissolve completely. DO NOT AUTOCLAVE. Cool to 44-47°C and use within 4 hours. Mix well before pouring. Description Various groups of bacteria or individual species are used to provide evidence of poor hygiene, inadequate processing or post-processing contamination of foods. Some bacteria, such as Escherichia coli, are present in the gastrointestinal tract of many animals, including humans, so their presence can be used to indicate potential faecal contamination. One of the most common groups of bacteria employed as indicator organisms by the food industry are the coliforms, which can be regarded as a subgroup within the Enterobacteriaceae. However, in an attempt to improve assessment of food safety and quality by the food industry, there has been a gradual move towards testing for total Enterobacteriaceae rather than limiting testing to coliforms. In processed foods, particularly foods subjected to heat treatment, these organisms can provide a reliable indication of process failure, under-processing or post-processing contamination. Mossel et al3 modified Violet Red Bile Lactose Agar by adding glucose to improve recovery of all Enterobacteriaceae. Later work by Mossel and Cowell4,5 demonstrated that lactose could be omitted, resulting in the formulation known as VRBGA.