ISP MEDIUM NO. 2 (YEAST MALT AGAR) (YM AGAR) HIMEDIA M424-500G

NAME : ISP MEDIUM NO. 2 (YEAST MALT AGAR) (YM AGAR) CODE : M424-500G BRAND : HIMEDIA PACKING : 500G MOHON CHAT TERLEBIH DULU UNTUK CEK KETERSEDIAAN BARANG DAN EXPIRED DATE For the isolation and cultivation of yeasts, moulds and other aciduric microorganisms Yeast Malt Agar is formulated as per Wickerham (1,2) for isolation and cultivation of yeasts, moulds and other aciduric microorganisms. Fungistatic materials such as sodium propionate and diphenyl are added to YM Agar to eliminate moulds and thus permits enumeration of yeasts from mixed population. YM Agar is also recommended by APHA (3). Wickerham suggested the use of Yeast Malt Broth (M425) as an enrichment medium for yeasts by adding a layer of sterile paraffin oil (about 1 cm) on the surface of inoculated broth. After the growth occurs it should be streaked on YM Agar to obtain isolated colonies of fermentative species. To isolate fermentative as well as oxidative strains, acidified YM Broth (M425) is placed on a rotary shaker for 1 or 2 days which favors development of yeast cells while the sporulation of moulds is prevented and yeasts can be isolated by streaking on YM Agar. Peptone serves as a source of carbon, nitrogen, long chain amino acids and other essential nutrients. Yeast extract supplies vitamin B complex nutrients and other growth factors. Malt extract serves as an additional source of carbon. Dextrose is the carbohydrate and energy source. To increase the selectivity, the media can be acidified by the addition of sterile10% Lactic Acid or by addition of 10% HCl, tartaric acid or 10% citric acid. Alternatively antibiotics (penicillin 20U/ml or streptomycin to a final concentration of 40mcg/ml) can be added. Acidified agar medium should not be reheated.