Murashige and Skoog Medium incl. Vitamins

Murashige amp; Skoog Basic Medium : Dehydrated powder premix for the preparation of a liquid medium, designed for in vitro plant tissue culture. This medium has originally been developed for callus cultures of Nicotiana tabacum. Nowadays it is a popular standard-medium for a wide variety of in vitrotechniques, suitable for a great number of plant species. The formulation (Tab. 1) contains macro- and microelements, vitamins and glycin as described by Murashige amp; Skoog (1962). Storage The mixture contains strong hygroscopic ingredients, a storage under dry conditions is necessary. Optimal storage temperature is between 2 – 8 °C. Dosage Typical: 4.41 g/l Preparation example 1. Dissolve powder in distilled water 2. Add further substances if needed (additives, carbon sources, auxins/cytokinins or other plant growth regulators)* 3. Adjust the pH using potassium hydroxide (KOH) / sodium hydroxide (NaOH) or hydrochloric acid (HCl) 4. If necessary, add gelling agent to obtain a solid media 5. Autoclave at 121 °C, 15 PSI