Tris (Hydroxymethyl) Aminomethane for Molecular Biology A 40% at 290 nm......max 0.03

PRODUCT CODE 998660 SYNONYMS (Tris buffer, Trometamol,2-Amino-2-(hydroxymethyl)-1 ,3- propanediol) C.I. NO. -- CASR NO. (77-86-1) ATOMIC OR MOLECULAR FORMULA NH2C(CH2OH)3 ATOMIC OR MOLECULAR WEIGHT 121.14 PROPERTIES Corrosive to copper, aluminium, brass. Combustible. NH2C(CH2OH)3 PARAMETER LIMIT Description White crystalline powder. Solubility 40% solution in HPLC water is clear and not more than 10 APHA in colour. Identification (By FTIR) Conforms. Minimum Assay (Acidimetric, on dried material) 99.8% Melting point 168-172°C pH (of 1M soln. in water) 10.5 – 12.0 MAXIMUM LIMIT OF IMPURITIES Loss on drying (at 105°C) 0.5% Maximum UV Absorbance (1M soln. in HPLC water) at 260 nm 0.04 Maximum UV Absorbance (40% soln.in HPLC water,1cm)at 290 nm 0.05 Insoluble matter Passes filter test. Chloride (Cl) 0.002% Sulphate(SO4) 0.0005% Iron (Fe) 0.0005% Heavy metals (as Pb) 0.0002% RNase Dnase,Protease and phosphatases None detected.