Loeffler Serum Medium Base 500gr

Untuk ketersediaan, harap hubungi terlebih dahulu Code: M1189 www.intralab.co.id No. telp: (0251) 8359110, 8311662 No. WA: 081808171907 Recommended for the cultivation of Corynebacterium diphtheriae. Directions Suspend 10.0 grams in 250 ml purified / distilled water at a temperature of 45oC. Add 750 ml of sterile Bovine serum, mix well and dispense into tubes. Coagulate and sterilize by inspissation for 15 minutes at 80 to 90oC or steaming at 100°C for 10-15 minutes. Principle And Interpretation Corynebacterium diphtheriae , also called as Klebs-Loeffler bacillus, is a gram-positive, non-encapsulated, non-sporulated, non-motile facultative anaerobe. It causes infection in humans, leading to diseased condition called diphtheria characterized by an inflammatory lesion and membranous exudates on the mucosa of the upper respiratory tract. C. diphtheriae may show abundant volutin in films from a moist Loeffler serum slope. Preliminary culture on Loeffler Agar is required to induce the characteristic production of abundant granules in C. diphtheriae. Loeffler Medium was originally devised by Loeffler (4) and was further modified by Perry and Petran (6) and Buck (1). Loeffler medium enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms especially from nose and throat. It also restores virulence and other identifying properties (microscopic and colonial) after they have been lost due to prolonged incubation or repeated subculturing. It is also used for demonstration of pigmentation and ascospores.