Mannitol Selenite Broth 500gr

Untuk ketersediaan, harap hubungi terlebih dahulu Code: M1534 www.intralab.co.id No. telp: (0251) 8359110, 8311662 No. WA: 081996981968 Mannitol Selenite Broth is used for selective enrichment of Salmonellae from clinical materials. Directions Suspend 4.0 grams of Part B in 1000 ml distilled water. Add 19.0 grams of Part A. Warm to dissolve the medium completely. Distribute in sterile test tubes. Sterilize in a boiling water bath or free flowing steam for 10 minutes. DO NOT AUTOCLAVE. Excessive heating is detrimental. Discard the prepared medium if large amount of selenite is reduced (indicated by red precipitate at the bottom of the tube). Caution: Sodium hydrogen selenite (Sodium biselenite) is very toxic, corrosive agent and causes teratogenicity. So it should be handled with great care. If there is contact, wash immediately with lot of water. Principle And Interpretation Selenite-containing media for the enrichment of Salmonella was first described by Guth (1). This medium was further modified by Leifson (2) for the enrichment and isolation of Salmonella from clinical specimens. Mannitol Selenite Broth is a selective enrichment medium, more or less similar to Leifson (2) enrichment medium, described by Hobbs and Allison (3) for the isolation of Salmonella Typhi and Salmonella Paratyphi B from clinical specimens. Mannitol Selenite Broth can also be used for the selective enrichment of Salmonella from water and foodstuffs.