Viva qGreen I Fluorescent Dye 20X in DMSO, 1ml, (SD1101), Vivantis

Description
Viva qGreen I Fluorescent Dye (equivalent to SYBR Green Dye) is a sensitive green fluorescent nucleic acid dye used for detection of double stranded DNA.
The dye is widely used in non-specific detection of amplification in quantitative realtime PCR (qPCR) experiments.
The detection is monitored by measuring the increase in fluorescence throughout the cycle.

Viva qGreen I fluorescent reporter offers distinct advantages. Besides to be double-strand DNA-specific, the fluorescent reporter
is also easy to be used and highly sensitive. Compared to other fluorescent dyes, Viva qGreen I Fluorescent Dye can be used to monitor
the amplification of any double-stranded DNA sequence. There is no probe required, which can reduce assay setup time as well as running costs.

Viva qGreen I Fluorescent Dye may have disadvantage to generate false positive signals due to the dye can also bind to any
double-stranded DNA sequences. However, the dye can equally good in detecting the non-specific products as well as primer dimer.
Therefore, it is very important to have welldesigned primers that do not amplify non-target sequences in qPCR reactions to prevent
overestimation of the target concentration.

Viva qGreen I Fluorescent Dye has an excitation and emission maxima of 494nm and 521nm, respectively.
The dye is compatible with PCR up to a point and it starts to inhibit the PCR reaction at very high concentration.


Application :
* Real-time PCR experiments – low level of quantitation
* Melt curve analysis
* Common DNA quantification
* Gene expression analysis
* Mass screening

More Info =
https://www.vivantechnologies.com/index.php?option=com_content&view=article&id=1274:viva-qgreen-i-fluorescent-dye-20x-in-dmso&catid=97:qpcr-dyes-&Itemid=129

No. Catalog : SD1101_viv

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