PSEUDOMONAS AGAR BASE 500GR | CM0559B CM-0559B OXOID

PSEUDOMONAS AGAR BASE 500GR | CM0559B CM-0559B OXOID Descriptions : Pseudomonas Agar Base is designed so that by the addition of the appropriate supplement (SR0102 or SR0103) the medium becomes selective for Pseudomonas aeruginosa or Pseudomonas spp. generally. The base medium is a modification of King’s A Medium1 in which magnesium chloride and potassium sulphate are present to enhance pigment production. Pseudomonas CN Supplement (SR0102) is recommended for the selective isolation of Pseudomonas aeruginosa. The formula of the supplement was described by Goto and Enomoto2 following the demonstration of cetrimide as a selective agent by Lowbury and Collins3. Goto and Enomoto showed that addition of nalidixic acid at 15µg/ml, while at the same time reducing the cetrimide content to 200mg, improved performance. The medium gave better recovery of Pseudomonas aeruginosa with enhanced pigment formation whilst strongly suppressing Klebsiella, Proteus and Providencia spp., the latter organisms being the troublesome contaminants of conventional cetrimide media. Pseudomonas CFC Supplement (SR0103) is recommended for the selective isolation of Pseudomonas spp. generally. Mead and Adams4 showed that reducing the cetrimide content to 10mg/ml allowed the growth of all pigmented and non- pigmented psychrophilic pseudomonads. To improve its selective action they added cephaloridine 50µg/ml) and fucidin (10µg/ml). This combination of agents gave a new and more specific medium for isolating pseudomonads from chilled foods and processing plants. Incubation should be carried out at 25-30°C for 48 hours.