Media Mikro Lactose Broth 500 gr, Oxoid CM0137

LACTOSE BROTH (LB), 500 GR, OXOID, CM0137B Merk : OXOID Negara : UK Catalog No. : CM0137B Packing : 500 GR (Plastic Bottle) OXOID QUALITY ASSURANCE PRODUCT SPECIFICATION LACTOSE BROTH CM0137 Typical Formula* ‘Lab-Lemco’ powder grams per litre 3.0 Peptone 5.0 Lactose 5.0 * adjusted as required to meet performance standards Directions Dissolve 13g in 1 litre of distilled water. Mix well and distribute into final containers with Durham tubes. Sterilize by autoclaving at 121°C for 15 minutes. Physical Characteristics Straw, free-flowing powder Colour on reconstitution - straw 1-2 Moisture level - less than 7% pH 6.9 ± 0.2 at 25°C Clarity - clear Microbiological Tests Using Optimum Inoculum Dilution Control Medium: Tryptone Soya Agar Medium is challenged with 10-100 colony-forming units Reactions after incubation at 37°C for 24 hours Escherichia coli ATCC 8739 Turbid growth, acid and gas Enterobacter aerogenes NCTC 9735 Turbid growth, acid with or without gas Check for gas at 24 hours, if no gas is visible continue incubation for 48 hours. Reactions after incubation at 37°C for 48 hours Escherichia coli ATCC 8739 Turbid growth, acid and gas Enterobacter aerogenes NCTC 9735 Turbid growth, acid and gas Enterococcus faecalis ATCC 19433 Turbid growth, acid and no gas Staphylococcus aureus ATCC 6538 Turbid growth, acid and no gas Pseudomonas aeruginosa ATCC 9027 Turbid growth, no reaction Salmonella abony NCTC 6017 Turbid growth, no reaction Salmonella nottingham NCTC 7832 Turbid growth, no reaction A satisfactory result is represented by visible growth. Acid production is detected by the addition of a few drops of bromocresol purple. A positive reaction is indicated by a colour change from purple to yellow