Description Where an AnaeroGen sachet is placed in a sealed jar, the atmospheric oxygen in the jar is rapidly absorbed with the simultaneous generation of carbon dioxide. This novel method differs from others commonly used in that the reaction proceeds with no evolution of hydrogen, and therefore, does not require a catalyst. Furthermore, water is not required to activate the reaction. When used as directed, the AnaeroGen sachet will reduce the oxygen level in the jar to below 1% within 30 minutes. The resulting carbon dioxide level will be between 9% and 13%. AnaeroGen was used in methodology for detecting bifidobacteria in meat and meat products in an investigation into the suitability of these organisms as indicators of faecal contamination. Components Each box contains: 10 AnaeroGen paper sachets which are individually foil packed. 1 Product Insert. The active component within each AnaeroGen sachet is ascorbic acid. Precautions This product is for in-vitro use only. As soon as the AnaeroGen paper sachet is exposed to the air, the reaction will start. It is therefore essential that the paper sachet is placed in the jar and the jar sealed within one minute. The reaction of the ascorbic acid with oxygen is exothermic. However, the temperature of the AnaeroGen paper sachet will not exceed 65°C. Storage Store at 2-25°C. Under these conditions, the AnaeroGen sachets will retain their reactivity until the expiry date given on the outer box and on the foil sachet. Directions AN0035 is designed for use in 3.5 litre jars. It is also suitable for use in the Oxoid Anaerobic Jar HP0011 and for other jars of similar capacity. AN0025 is designed for use in 2.5 litre jars such as the new Oxoid AnaeroJar AG0025 and other jars of similar capacity.
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