Plate Count Agar / Standard Methods Agar 500 G, HIMEDIA

Media Mikrobiologi Plate Count Agar / Standard Methods Agar adalah media mikrobiologi yang direkomendasikan untuk analisa jumlah bakteri dalam sampel makanan, minuman, air, dll Kemasan / Berat netto : 500 gram Plate Count Agar (Standard Methods Agar) M091 Plate Count Agar is recommended for the determination of plate counts of microorganisms in food, water, waste water and also from clinical samples. Composition** Ingredients Gms / Litre Casein enzymic hydrolysate 5.000 Yeast extract 2.500 Dextrose 1.000 Agar 15.000 Final pH ( at 25C) 7.00.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 23.5 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Mix well and pour into sterile Petri plates. Principle And Interpretation Plate Count Agar is formulated as described by Buchbinder et al (1) which is recommended by APHA (2,3,4) and FDA (5). Casein enzymic hydrolysate provides amino acids and other complex nitrogenous substances. Yeast extract supplies Vitamin B complex. APHA recommends the use of pour plate technique. The samples are diluted and appropriate dilutions are added in Petri plates. Sterile molten agar is added to these plates and plates are rotated gently to ensure uniform mixing of the sample with agar. The poured plate count method is preferred to the surface inoculation method, since it gives higher results. Plate Count Agar is also suitable for enumerating bacterial count of sterile rooms