Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit

Xanthine Oxidase (XOD) Activity Fluorometric Assay Kit Cat.No.:E-BC-F019 Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm) Detection method: Fluorescence method Detection principle Hypoxanthine are oxidized by xanthine oxidase (XOD) to produce xanthine and super oxygen anion, which will quickly converte to hydrogen peroxide in the system, and then, in the role of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe to fluorescent substance. By measuring the fluorescence value, the corresponding the activity of xanthine oxidase can be calculated. Performance characteristics Synonyms XOD Sample type Serum, plasma, animal tissue Sensitivity 0.01 U/L Detection range 0.01 -1.2 U/L Detection method Fluorescence method Assay type Enzyme Activity Assay time 25 min Precision Average inter-assay CV: 9%Average intra-assay CV: 4.1% Other instruments required Vortex mixer, Centrifuge Storage -20℃ Valid period 6 months