OXOID CM0129 Tryptone Soya Broth 500 gr Oxoid CM0129B

TRYPTONE SOYA BROTH 500 GR| CM0129B OXOID Description A highly nutritious versatile medium which is recommended for general laboratory use. Due to the inclusion of both Tryptone and Soya Peptone, the medium will support a luxuriant growth of many fastidious organisms without the addition of serum, etc. Tryptone Soya Broth conforms to formulations detailed in various international pharmacopoeias1,4,5,6 . Technique Aerobic Cultivation Tryptone Soya Broth may be used for the cultivation of aerobes and facultative anaerobes, including some fungi. Cultures should be examined at frequent intervals, as maximum growth is reached earlier than with less nutritious media and the phase of decline consequently begins sooner. Anaerobic Cultivation The addition of a small amount of agar renders the broth suitable for the cultivation of obligatory anaerobes, such as Clostridium spp.. For this purpose, the broth (with added agar) should be used soon after sterilisation, or, heated and cooled just before inoculation. Blood Culture The superior growth-promoting properties of Tryptone Soya Broth make it especially useful for the isolation of organisms from blood or other body fluids. Anticoagulants such as `liquid (sodium polyanethyl sulphonate) or sodium citrate may be added to the broth prior to sterilisation. Five to 10ml of blood may be added to 50ml of medium. Tryptone Soya Broth is especially suitable for the tube dilution method of antibiotic susceptibility testing. Oxoid laboratory tests have shown that Tryptone Soya Broth has a greater ability to resuscitate heated spores of Geobacillus stearothermophilus than Dextrose Tryptone Broth. Tryptone Soya Broth is recommended in the USP (Casein soya bean digest broth) for the recovery of organisms after sterilisation processes1. A positive result is revealed after 24-48 hours incubation at 55C, by a heavy growth of organisms causing turbidity in the broth. Roche Products Ltd. Welwyn Garden City, Herts.